Optimization of multibiosensor operation for inhibitory analysis of toxins

Мова статті: 
Англійська
Автор(и) статті: 
Soldatkin O. O., Pavluchenko O. S., Kukla O. L., Arkhypova V. M., Dzyadevych S. V., Soldatkin O. P., El'skaya A. V.
Вихідні данні статті: 
Biopolymers and Cell. 2008; 24(6): 494-502
Назва журналу: 
Biopolymers and Cell
Рік публікації: 
2008
Volume: 
24
Issue: 
6
Ключові слова: 
multibiosensor, immobilized enzymes, pH-sensitive field-effecttransistors, inhibitory analysis, toxins
Ключове слово: 
multibiosensor
Ключове слово: 
immobilized enzymes
Ключове слово: 
pH-sensitive field-effecttransistors
Ключове слово: 
inhibitory analysis
Ключове слово: 
toxins

The operation of highly sensitive and selective multibiosensor based on different immobilized enzymes as bioselective elements and the matrix of pH-sensitive field effect transistors as transducers has been investigated. To develop bioselective elements of multibiosensor, the enzymes acetylcholinesterase, butyryl-cholinesterase, urease, glucose oxidase, and three-enzyme system invertase-mutarotase-glucose oxidase with high sensitivity to toxins were used. The optimal concentrations of substrates for inhibitory analysis application were chosen as follows: 10 mM acetylcholine, 5 mM butyrylcholine, 5 mM urea, 5 mM sucrose, and 2 mM glucose. The incubation time of multibiosensor in toxic solution was 20 min. No cross-influence of substrates in all the enzyme systems used was found. The inhibitory influence of separate toxins and their mixture on the bioselective elements of multibiosensor was studied.

Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov