Optical fibre biosensors using enzymatic transducers to monitor glucose

Мова статті: 
Англійська
Автор(и) статті: 
P.J.Scully, L.Betancor, J.Bolyo, S.Dzyadevych, J.M.Guisan, R.Fernandez-Lafuente, N.Jaffrezic-Renault, G.Kuncova, V.Matejec, B.O'Kennedy, O.Podrazky, K.Rose, L.Sasek, J.S.Young
Вихідні данні статті: 
Measurement Science and Technology.- Volume 18, Issue 10, 1 October 2007, Pages 3177-3186
Назва журналу: 
Measurement Science and Technology
Рік публікації: 
2007
Volume: 
18
Issue: 
10
Ключові слова: 
Bioreactor; Biosensor; Enzyme; Fluorescence; Fluorophore; Glucose; Glucose oxidase; Hybrid coating; Optical fibre sensor; Oxygen; POF; Polymer; Ruthenium
Ключове слово: 
Bioreactor
Ключове слово: 
Biosensor
Ключове слово: 
Enzyme
Ключове слово: 
Fluorescence
Ключове слово: 
Fluorophore
Ключове слово: 
Glucose
Ключове слово: 
Glucose oxidase
Ключове слово: 
Hybrid coating
Ключове слово: 
Optical fibre sensor
Ключове слово: 
Oxygen
Ключове слово: 
POF
Ключове слово: 
Polymer
Ключове слово: 
Ruthenium
The construction and performance of a novel enzyme based optical sensor for in situ continuous monitoring of glucose in biotechnological production processes is presented. Sensitive optical coatings are formed from inorganic-organic hybrid polymers (ORMOCERsORMOCER: Trademark of Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V. in Germany.) combined with a flurophore (ruthenium complex) and an enzyme, and applied to lenses, declad polymer optical fibre (POF) and polymer clad silica fibre (PCS). The enzyme, glucose oxidase, catalyzes oxidization of glucose to gluconic acid by depleting oxygen. Oxygen consumption is determined by measuring the fluorescence lifetime of metal organic ruthenium complexes which are quenched by oxygen. The coatings developed were designed to adhere to glass and polymer surfaces, to be compatible with enzymes and ruthenium complexes, and were demonstrated both as double- and single-layer structures. The sensor response to gaseous oxygen, dissolved oxygen and dissolved glucose was measured via fluorescence lifetime changes. A best detection limit of 0.5% (vol) has been determined for gaseous O2 with selected ORMOCER sensing layers. Glucose concentrations were measured to a detection limit of 0.1 mmol L -1 over a range up to 30 mmol L-1. The sensor was usable for 30 days in a bioreactor. The opto-electronic instrumentation and performance in laboratory bioreactors and in an industrial reactor are evaluated. © 2007 IOP Publishing Ltd.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov