Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions

Мова статті: 
Англійська
Автор(и) статті: 
O.O. Soldatkin, I.S. Kucherenko, V.M. Pyeshkova, A.L. Kukla, N. Jaffrezic-Renault, A.V. El'skaya, S.V. Dzyadevych, A.P. Soldatkin
Вихідні данні статті: 
Bioelectrochemistry vol.83, pp. 25-30 (Published Feb 2012, Available online 18 August 2011)
Назва журналу: 
Bioelectrochemistry
Рік публікації: 
2012
Volume: 
83
Ключові слова: 
biosensor; heavy metal ions; inhibitory analysis; enzyme; conductometric transducer
Ключове слово: 
biosensor
Ключове слово: 
heavy metal ions
Ключове слово: 
inhibitory analysis
Ключове слово: 
enzyme
Ключове слово: 
conductometric transducer

A differential pair of planar thin-film interdigitated electrodes, deposited on a ceramic pad, was used as a conductometric transducer. The three-enzyme system (invertase, mutarotase, glucose oxidase), immobilized on the transducer surface, was used as a bioselective element. The ratio between enzymes in the membrane was found experimentally considering the highest biosensor sensitivity to substrate (sucrose) and heavy metal ions. Optimal concentration of sucrose for inhibitory analysis was 1.25 mM and incubation time in the investigated solution amounted to 10–20 min. The developed biosensor demonstrated the best sensitivity towards ions Hg2+ and Ag+. A principal possibility of the biosensor reactivation either by EDTA solution after inhibition with silver ions or by cysteine solution after inhibition with mercury ions was shown.

Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
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Дизайн: Інститут високих технологій
Ivan Ivanov