Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals

Мова статті: 
Англійська
Автор(и) статті: 
E.Soy, V.Arkhypova, O.Soldatkin, M.Shelyakina, S.Dzyadevych, J.Warzywoda, A.Sacco Jr., B.Akata
Вихідні данні статті: 
Material Science and Engineering C V.32.- P. 1835-1842 2012
Назва журналу: 
Material Science and Engineering C
Рік публікації: 
2012
Volume: 
32
Issue: 
7
Ключові слова: 
Butyrylcholinesterase; Ion sensitive field effect transistor; Urea; Urease; Zeolite acidity; Zeolite Beta
Ключове слово: 
Butyrylcholinesterase
Ключове слово: 
Ion sensitive field effect transistor
Ключове слово: 
Urea
Ключове слово: 
Urease
Ключове слово: 
Zeolite acidity
Ключове слово: 
Zeolite Beta
Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Brønsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to ~ 2 and ~ 5 times, respectively; and correlated with the amount of Brønsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Brønsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Brønsted acidity can improve the performance of ISFET-based biosensors.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
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Дизайн: Інститут високих технологій
Ivan Ivanov