Direct evidence of advantage of using nanosized zeolite Beta for ISFET-based biosensor construction

Мова статті: 
Англійська
Автор(и) статті: 
Esin Soy, Sezin Galioglu, Oleksandr O. Soldatkin, Sergei V. Dzyadevych, Juliusz Warzywoda, Albert Sacco Jr., Burcu Akata
Вихідні данні статті: 
Journal of Nanoparticle Research Volume 15, Issue 5, 2013
Назва журналу: 
Journal of Nanoparticle Research
Рік публікації: 
2013
Volume: 
15
Issue: 
5
Ключові слова: 
Butyrylcholinesterase; ISFETs; Nanoparticles; Urease; Zeolite Beta
Ключове слово: 
Butyrylcholinesterase
Ключове слово: 
ISFETs
Ключове слово: 
Nanoparticles
Ключове слово: 
Urease
Ключове слово: 
Zeolite Beta
Analytical characteristics of urease- and butyrylcholinesterase (BuChE)- based ion sensitive field-effect transistor (ISFET) biosensors were investigated by the incorporation of zeolite Beta nanoparticles with varying Si/Al ratios. The results obtained by the zeolite-modified ISFET transducers suggested that the Si/Al ratio strongly influenced the biosensor performances due to the electrostatic interactions among enzyme, substrate, and zeolite surface as well as the nature of the enzymatic reaction. Using relatively small nanoparticles (62.7 ± 10, 76.2 ± 10, and 77.1 ± 10 nm) rather than larger particles, that are widely used in the literature, allow us to produce more homogenous products which will give more control over the quantity of materials used on the electrode surface and ability to change solely Si/Al ratio without changing other parameters such as particle size, pore volume, and surface area. This should enable the investigation of the individual effect of changing acidic and electronic nature of this material on the biosensor characteristics. According to our results, high biosensor sensitivity is evident on nanosize and submicron size particles, with the former resulting in higher performance. The sensitivity of biosensors modified by zeolite particles is higher than that to the protein for both types of biosensors. Most significantly, our results show that the performance of constructed ISFET-type biosensors strongly depends on Si/Al ratio of employed zeolite Beta nanoparticles as well as the type of enzymatic reaction employed. All fabricated biosensors demonstrated high signal reproducibility and stability for both BuChE and urease. © 2013 Springer Science+Business Media Dordrecht.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov