Development of trypsin biosensor based on ion sensitive field-effect transistors for proteins determination

Мова статті: 
Англійська
Автор(и) статті: 
M.Marrakchi, S.V.Dzyadevych, O.A.Biloivan, C.Martelet, P.Temple, N.Jaffrezic-Renault
Вихідні данні статті: 
Material Science and Engineering C.- Volume 26, Issue 2-3, March 2006, Pages 369-373
Назва журналу: 
Material Science and Engineering C
Рік публікації: 
2006
Volume: 
26
Issue: 
2-3
Ключові слова: 
Biosensor; Ion sensitive field effect transistor; Protein; Trypsin
Ключове слово: 
Biosensor
Ключове слово: 
Ion sensitive field effect transistor
Ключове слово: 
Protein
Ключове слово: 
Trypsin
This new biosensor for protein determination is based on a possibility of translation of either a proton or hydroxyl ion arising during protein hydrolysis in the presence of trypsin by means of ion selective field effect transistor (ISFET). The conditions of trypsin immobilization and main biosensor characteristics were optimized using hydrolysis reaction of nalpha-benzoyl-l- arginine ethyl ester hydrochloride (BAEE). The trypsin was immobilized on the ISFET surface using a co-reticulation process between the enzyme and BSA (4% trypsin, 6% BSA) in saturated glutaraldehyde vapour. The limit of detection of BAEE with this biosensor is 0.5 mM with a linear dynamics range from 0.5 to 5 mM, and sensitivity is about 6 mV/mM. The time of biosensor response was 5-7 min. The possibility of the application of developed biosensor for detection of small penta-peptide, which is used in some cosmetic products, has been demonstrated. © 2005 Elsevier B.V. All rights reserved.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov