Development and optimization of novel conductometric bi-enzyme biosensor for L-arginine determination

Мова статті: 
Англійська
Автор(и) статті: 
O.Y.Saiapina, N.Jaffrezic-Renault, A.P.Soldatkin, S.V.Dzyadevych
Вихідні данні статті: 
Talanta 92.- P.58-64 2012
Назва журналу: 
Talanta
Рік публікації: 
2012
Volume: 
92
Ключові слова: 
l-Arginine determination; Conductometric biosensor; Arginase; Urease; Quality-control analysis
Ключове слово: 
l-Arginine determination
Ключове слово: 
Conductometric biosensor
Ключове слово: 
Arginase
Ключове слово: 
Urease
Ключове слово: 
Quality-control analysis
A highly sensitive conductometric biosensor for l-arginine determination was developed by exploiting the unique biorecognition capacities of two enzymes of urea cycle - arginase (E.C. 3.5.3.1) and urease (E.C. 3.5.1.5). The enzymes were co-immobilized in a single bioselective membrane on the working sensor, while a lysine rich bovine serum albumin (BSA) membrane was immobilized on the reference sensor, allowing differential measurements. The optimum percentage ratio of arginase and urease within the bioselective membrane was determined when the biosensor sensitivity to l-arginine and urea was optimum. Analytical characteristics of the conductometric biosensor for l-arginine determination were compared for two types of enzyme immobilization (cross-linking with glutaraldehyde (GA) and entrapment in the polymeric membrane). The optimum features in terms of the sensitivity, the linear range, and the detection limit (4.2 μS/mM, 0.01-4mM, and 5.0 × 10(-7)M, respectively) were found for l-arginine biosensor based on enzyme cross-linking with GA. A quantitative determination of l-arginine in the real sample (a drinkable solution "Arginine Veyron") gave a satisfactory result compared to the data provided by the producer (a relative error was 4.6%). The developed biosensor showed high operational and storage stability.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
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Дизайн: Інститут високих технологій
Ivan Ivanov