Application of different zeolites to improve characteristics of pH-FETs biosensor based on immobilized urease

Мова статті: 
Англійська
Автор(и) статті: 
O.O.Soldatkin, I.S.Kucherenko, M.K.Shelyakina, E.Soy, K.Kirdeciler, S.Öztürk, B.Akata, S.V.Dzyadevych, A.P.Soldatkin
Вихідні данні статті: 
Electroanalys.- 2013.-Volume 25, Issue 2, pages 468–474
Назва журналу: 
Electroanalys
Рік публікації: 
2013
Volume: 
25
Issue: 
2
Ключові слова: 
Biosensors; pH-FET; Urease; Zeolites
Ключове слово: 
Biosensors
Ключове слово: 
pH-FET
Ключове слово: 
Urease
Ключове слово: 
Zeolites
Different modifications of the zeolites Na+-Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co-immobilized with bovine serum albumin on the surface of a pH-FET. It was shown that the biosensors modified with the zeolites H+-Beta30 and H+-Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H+-Beta30 and H+-Beta50 in the bioselective membrane was 15 %. Different variants of co-immobilization of urease and zeolite H+-Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H+-Beta30 for urease immobilization in the bioselective membrane.
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov