Acetylcholinesterase-based conductometric biosensor for determination of aflatoxin B1

Мова статті: 
Англійська
Автор(и) статті: 
O.O. Soldatkin, O.S. Burdak, T.A. Sergeyeva, V.M. Arkhypova, S.V. Dzyadevych, A.P. Soldatkin
Вихідні данні статті: 
Sensors and Actuators, B: Chemical Volume 188, 2013, Pages 999-1003
Назва журналу: 
Sensors and Actuators, B
Рік публікації: 
2013
Volume: 
188
Ключові слова: 
Acetylcholinesterase; Aflatoxin B1; Biosensor; Conductometric transducer; Environmental monitoring; Inhibitory analysis
Ключове слово: 
Acetylcholinesterase
Ключове слово: 
Aflatoxin B1
Ключове слово: 
Biosensor
Ключове слово: 
Conductometric transducer
Ключове слово: 
Environmental monitoring
Ключове слово: 
Inhibitory analysis
The theoretical and experimental studies were carried out, which resulted in the development of acetylcholinesterase-based conductometric biosensor for determination of aflatoxin B1. We established a linear range of substrate measurement (0.25-1 mM) and optimal concentration of acetylcholine for inhibitory analysis (1 mM). The sensitivity of developed biosensor to aflatoxin B1 was tested, the calibration curve was plotted for the dependence of residual activity of the acetylcholinesterase-based bioselective element on the toxin concentration. Detection limit of aflatoxin B1 was 0.05 μg/ml. It was shown that the developed biosensor was characterized by high signal reproducibility of direct determination of the main substrate as well as of the inhibitory analysis of aflatoxin B1 (the error of detection was no more than 7%)
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov