(1)H, (13)C, and (15)N chemical shifts assignments for human endothelial monocyte-activating polypeptide EMAP II

Мова статті: 
Автор(и) статті: 
D.Lozhko, J.Stanek, K.Kazimierczuk, A.Zawadzka-Kazimierczuk, W. Kozminski, I.Zhukov and A.Kornelyuk
Вихідні данні статті: 
Biomolecular NMR Assignments, 2012 Mar 6
Назва журналу: 
Biomolecular NMR Assignments
Рік публікації: 
Ключові слова: 
Multidimensional NMR spectroscopy, Random sampling, Sequence-specific backbone assignment, Side chain assignments, Cytokines, EMAP II
Ключове слово: 

Endothelial and monocyte-activating polypeptide II (EMAP II) is a cytokine that plays an important role in inflammation, apoptosis and angiogenesis processes in tumour tissues. Structurally, the EMAP II is a 169 amino acid residues long C-terminal domain (residues 147-312) of auxiliary tRNA binding protein p43. In spite of existence in pdb databank of two X-ray structures there are some important aspects of EMAP II cytokine function which are still not fully understood in detail. To obtain information about 3D structure and backbone dynamic processes in solution we perform structure evaluation of human EMAP II cytokine by NMR spectroscopy. The standard approach to sequence-specific backbone assignment using 3D NMR data sets was not successful in our studies and was supplemented by recently developed 4D NMR experiments with random sampling of evolution time space. Here we report the backbone and side chain (1)H, (13)C, and (15)N chemical shifts in solution for recombinant EMAP II cytokine together with secondary structure provided by TALOS + software

Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
ІНСТИТУТ ВИСОКИХ ТЕХНОЛОГІЙ Матеріали дозволено використовувати на умовах GNU FDL без незмінюваних секцій та Creative Commons Attribution/Share-Alike
Дизайн: Інститут високих технологій
Ivan Ivanov