Anatoliy I. Dragan

Візитка
Стан: 
Викладач
Науковий ступінь: 
к. б. н.
Посада: 
Доцент
Кафедра чи НДЧ-підрозділ: 
Кафедра молекулярної біології, біотехнології та біофізики
RESEARCH INTERESTS
 

   Principles of structural organization of biological macromolecules, their interactions and cooperation in formation of specific protein-protein and protein/DNA complexes. The energetics of bio-molecular structures, the forces determining thr folding and stability of biopolymers, the mechanisms of their association with partners.  Understanding of the physical basis of such biological systems is important not only for fundamental research in biology but also for practical exploitation of these fundamental achievements, particularly in engineering various analytical applications.

    A recent interest has been the theory of Plasmon Resonance Metal-Enhanced Fluorescence (MEF) and its application for rapid analysis of drugs, proteins and DNA; in particular, the creation of new sensor platforms in medicine and biology based on advanced spectroscopy and nanotechnologies. 

 

EXPERTISE

Advanced optical spectroscopy, biological microcalorimetry, Metal Enhanced Fluorescence. Expertise in the structure and energetics/thermodynamics of biological macromolecules (proteins, protein-protein complexes, DNA and DNA-protein complexes).

EDUCATION

1985
 
Ph.D.  in Biochemistry
National University, Kiev, Ukraine
1976
 
MS in Physics
National University, Department of Optics and Spectroscopy, Faculty of Physics, Kiev, Ukraine
 
 
 

RESEARCH AND PROFESSIONAL EXPERIENCE

Academic positions

 1977-1988  
 
Research Scientist, Senior Research Scientist, Department of General and Molecular Genetics, National University, Kiev, Ukraine.
 
1988-2000
 
Associate Professor (tenured), Department of General and Molecular Genetics, National University, Kiev, Ukraine.
1999-2000
 
Visiting Scientist in the Biophysics Laboratories, University of Portsmouth, UK, in the framework of a European Union INTAS Program Collaborative Grant.
 
2000
 
Visiting Scientist in the Department of Biology, Johns Hopkins, University, Baltimore, MD, USA.
 
2001-2008
 
Research Scientist, Department of Biology, Johns Hopkins University, Baltimore, MD, USA
 2008-2010 
 
Senior Research Associate, Institute of Fluorescence, University of Maryland Biotechnology Institute, Baltimore, MD.
 2010-2013 
 
Research Assistant Professor, the Institute of Fluorescence, Department of Chemistry and Biochemistry, UMBC, Baltimore, MD
 
 
AWARDS/HONORS
 
1994
 
Yaroslav Mudryi Award of the Academy of Science of High School of Ukraine for a series of works on chromatin structure and function, and for outstanding contributions to science and education.
1995
 
Award of the International Soros Science Education Program   (ISSEP): in recognition and appreciation of outstanding contributions to science education at the university level. Elected and named as a Soros Associate Professor.

                                                                      

PROFESSIONAL MEMBERSHIPS

Member of American Biophysical Society;

Member of Ukrainian Biophysical Society.

 

TEACHING EXPERIENCE

1988 – 2000: Associate Professor (tenured position) at the Department of General and Molecular Genetics, Faculty of Biology, Taras Shevchenko National University, Kiev, Ukraine.

Total hours of teaching: 700 hours/year

2010: Research Assistant Professor at the Institute of Fluorescence, UMBC

  • Optical spectroscopy selected lectures for graduate and undergraduate students.

 

COMPLETED PROJECTS:

1997-1998
 
Principal Investigator. “Structure and Function of the Chromosomal Activator Protein HMG1” funded by the European Union INTAS Program (INTAS-94-0280)
2001-2004
 
Research Scientist. “Forces stabilizing protein structure” supported by National Institute of Health Grant (NIH Grant GM48036-10)
2005-2008
 
Co-Principal Investigator on the project “Energetics of DNA recognition by the bZIP transcription factors” supported by the National Science Foundation (NSF Grant MCB0519381)
 
2008-2011
 
Investigator on the project “Ultra Fast DNA quantitation assays” supported by MedImmune LLC
 
2009
 
Present: Investigator on the project “Stability and self-association of IgG proteins”, supported by MedImmune LLC
 
2009
 
Present: Investigator on the project “Ultra-Fast and Ultra-Sensitive Anthrax toxin detection assay based on Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF-ELISA)” supported by the Biological Defense Research Directorate

 

INVENTIONS

  1. Dragan, A.I. and Khrapunov, S.N. “Method of mutagenic treatment of plants”. Inventor’s certificate issued by the State Committee on Matters of Inventions and Discoveries of the USSR, No. 1765926. Priority date: July 12, 1990, Registration date: June 12, 1992.

  2. Geddes, C.D. and Dragan, A.I. “Tunable Metal-Enhanced Fluorescence Enhancement Factors” Provisional Application for Patent. Docket Number: UMBC2611CG. Date: 2010-05-11. Registration Number: 44903.

 

Review Services

Reviewer for the journals: Nucleic Acids Research, Journal of Molecular Biology,Biophysical Chemistry.
 

List of publications

Published papers, invited manuscripts and books.

Books and chapters:

 

  1. Crane-Robinson, C., Dragan, A. I. and Read, C. M. (2009) Defining the Thermodynamics of Protein/DNA Complexes and Micro-calorimetry. Chapter in book: Methods in Molecular Biology, 543, 625-651.

 

  1. Favicchio, R., Dragan, A. I., Kneale, G and Read C. M. (2009) Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions. Chapter in book: Methods in Molecular Biology, 543, 589-611.

 

  1. Dragan, A. I. & Privalov, P. L.  (2008) Use of fluorescence energy transfer (FRET) in studying protein-induced DNA bending (Review).  Chapter in book: Methods in Enzymology, Fluorescence Spectroscopy, volume 450, 185-199.

 

  1. Privalov, P.L & Dragan. A.I. (2007) Heat capacity microcalorimetry in studying the energetic bases of proteins and their complexes. Chapter in Protein Structures: Methods in Protein Structure and Stability Analysis. ; Ed. V. Uversky, Nova Science Publisher, Inc. NY.

 

Papers:

 

  1. Dragan, AI., Mali, B., and Geddes, CD., (2013). Wavelength-dependent Metal-Enhanced Fluorescence using synchronous spectral analysis, Chemical Physics Letters, 556, 168-172.

 
  1. Shukla, H., Vaitiekunas, P., Majumdar, A.K., Dragan, A.I., Dimitriadis, E.K., Kotova, S., Crane-Robinson, C. and Privalov, P.L. (2012) The linker of the IRF-3 transcription factor is not unfolded, Biochemistry , July 20 [Epub ahead of print].

 
  1. Dragan, A.I., Pavlovic, R., McGivney, J.B., Casas-Finet, J.R., Bishop, E.S., Strouse, R.J., Schenerman, M.A., Geddes, C.D. (2012) SYBR Green I: Fluorescence Properties and Interaction with DNA. J. Fluorescence, 22(4), 1189-1199.

 
  1. Dragan, A. I., Bishop, E. S., Casas-Finet, J. R., Strouse, R. J., McGivney, J., Schenerman, M. A., and Geddes, C. D., (2012). Distance Dependence of Metal-Enhanced Fluorescence, Plasmonics - In Press.

 
  1. Dragan, A.I. and Geddes, C.D. (2012) Metal-Enhanced Fluorescence: The     Role of Quantum Yield, Q0, in Enhanced Fluorescence. Applied Physical     Letters, 100, 093115.

 
  1. Dragan, A.I., Albrecht, M.T., Pavlovic, R., Keane-Myers, A.M. and Geddes, C.D. (2012) Ultra-Fast pg/ml Anthrax toxin (PA) detection assay based on Microwave Accelerated Metal-Enhanced Fluorescence, Analytical Chemistry, 425(1), 54-61.

 
  1. Feng, C., Stamatos, N. M., Dragan, A.I., Medvedev, A., Whitford, M., Zhang, L., Song C., Rallabhandi, P., Nhu, Q., Vogel, S. N., Geddes C. D., Cross, A. S. (2012), Sialyl Residues Modulate LPS-Mediated Signaling through the Toll-Like Receptor 4 Complex. PLOS One, 7(4),e32359.

 
  1. Dragan, A.I. and Geddes, C.D. (2011) Excitation Volumetric Effect (EVE) in Metal-Enhanced Fluorescence. Physical Chemistry Chemical Physics, 13, 3831-3838.

 
  1. Goldberg, K., Elbaz, A., Zhang, Y., Dragan, A., Marks, R., and Geddes, C. D., (2011). Mixed-metal substrates for applications in Metal-Enhanced Fluorescence, Journal of Materials Chemistry, 21, 6179-6185.

 
  1. Dragan, A.I., Pavlovic, R. and Geddes, C.D. (2011) Rapid Catch and Signal (RCS) platform technology: Multiplex three color, microwave-accelerated metal-enhanced fluorescence 20 second DNA assay. Biophysical Journal, 100(3), 137a – 138a.

 
  1. Mishra, H., Dragan, A.I. and Geddes, C.D. (2011) UV to NIR Surface Plasmon Coupled and Metal-Enhanced Fluorescence Using Indium Thin Films: Application to Intrinsic (Label-less) Protein Fluorescence Detection. The Journal of Physical Chemistry C, 115(35), 17227-17236.  

 
  1. Dragan, A.I., Golberg, K., Elbaz, A., Marks, R., Zhang, Y. and Geddes, C.D. (2011) Two-color, 30 second Microwave-Accelerated Metal-Enhanced Fluorescence DNA assays: A new Rapid Catch and Signal (RCS) technology. Journal of Immunological Methods, 366, 1-7.

 
  1. Privalov, P.L., Dragan, A.I. and Crane-Robinson, C.R. (2011) Interpreting protein/DNA interactions: distinguishing specific from non-specific and electrostatic from non-electrostatic components. Nucleic Acid Research, Survey and Summary, 39(7), 2483-2491.

 
  1. Dragan, A.I., Bishop, E.S., Casas-Finet,J.R., Strouse, R.J., Schenerman, M.A. and Geddes, C.D. (2010) Metal-Enahanced PicoGreen Fluorescence: Application for dsDNA Quantification. Analytical Biochemistry, 396(1), 8-12.

 
  1. Dragan, A.I. and Geddes, C.D. (2010) Indium nanodeposits: A substrate for Metal-Enhanced Fluorescence in the UV spectral region. Journal of Applied Physics, 108, 094701.

 
  1. Dragan, A.I., Bishop, E.S., Casas-Finet, J.R., Strouse, R.J., Schenerman, M.A. and Geddes, C.D. (2010) Metal-enhanced PicoGreen® fluorescence: Application to fast and ultra-sensitive pg/ml DNA quantitation. Journal of Immunological Methods., 362 (1-2), 95-100.

 
  1. Dragan, A.I., Casas-Finet, J.R., Bishop, E.S., Strouse, R.J., Schenerman, M.A. and Geddes, C.D. (2010) “Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding. Biophysical Journal, 99 (9), 3010-3019.

 
  1. Zhang, Y.X., Mandeng, L.N., Bondre, N., Dragan, A.I. and Geddes, C.D. (2010) Metal-enhanced fluorescence from silver-SiO2-silver nanoburger structures. Langmuir, 26 (14), 12371-12376.

 
  1. Zhang, Y.X., Dragan, A.I. and Geddes, C.D. (2010) Metal-enhanced fluorescence from tin nanostructured surfaces. J. Appl. Phys., 107(2), 024302.

 
  1. Carrillo, R.J, Dragan, A.I. and Privalov, P.L. (2010) Stability and DNA-binding ability of the bZIP dimers formed by the ATF-2 and c-Jun transcription factors. J. Mol. Biol., 369 (2), 431-440.

 
  1. Dragan, A.I., Bishop,E.S., Casas-Finet,J.R., Schenerman, M.A. and Geddes, C.D. (2009) Metal-Enhanced Ethidium Bromide Emission: Application to dsDNA Detection., Chem. Phys. Letters, 480 (4-6), 296-299.

 
  1. Zhang, Y., Dragan, A.I. and Geddes, C.D. (2009) Broad wavelength range metal-enhanced fluorescence using nickel nanodeposits. The Journal of Physical Chemistry C., 113 (36), 15811-16.

 
  1. Dragan, A.I., Zhang, Y. and Geddes, C.D. (2009) Voltage-Gated Metal-Enhanced Fluorescence II: Effects of Fluorophore Concentration on the Magnitude of the Gated-Current. J. Fluoresc., 19, 369–374.

 
  1. Zhang, Y., Dragan, A.I., Aslan, K. and Geddes, C.D. (2009) Wavelength - Dependence of Metal-Enhanced Fluorescence. Journal of Physical Chemistry C.,113 (28), 12095-12100.

 
  1. Pribik, R, Dragan, A.I., Zhang, Y. and Geddes, C.D. (2009) Metal_Enhanced     Fluorescence (MEF): Physical characterization of silver-island films and exploring sample geometries. Chemical Physics Letters, 478 (1-3), 70-74.

 
  1. Privalov, P.L., Dragan, A.I. and Crane-Robinson, C. (2009) The cost of DNA Bending. Trends in Biochemical Sciences, 34 (9), 464-470.

 
  1. Dragan, A. I. Russel, D.J. and Privalov, P. L.  (2009) DNA Hydration Studied by Pressure Perturbation Scanning Microcalorimetry, Biopolymers. 91(1):95-101.

 
  1. Dragan, A.I., Geddes, C.D. (2009) Plasmonic electricity: A Digital form of Metal-Enhanced Fluorescence. Biophysical Journal, 96 (3), 45a-46a.

 
  1. Dragan, A.I., Carrillo, R., Gerasimova, T.I., Privalov, P.L. (2008) Assembling the human IFN-beta enhanceosome in solution. J Mol Biol., Dec 12; 384(2), 335-48.

 
  1. Dragan, A. I. & Privalov, P. L.  (2008) Use of fluorescence energy transfer (FRET) in studying protein-induced DNA bending (Review).  Chapter in book: Methods in Enzymology, Fluorescence Spectroscopy, volume 450, 185-199.

 
  1. Dragan, A.I., Hargreaves, V.V., Makeyeva, E.N. & Privalov, P.L. & (2007) Mechanisms of activation of interferon regulator factor 3: the role of C-terminal domain phosphorylation in IRF-3 dimerization and DNA binding. Nucleic Acids Res. 35(11), 3525-3534.

 
  1. Privalov, P.L., Dragan, A.I., Crane-Robinson, C., Breslauer, K.J., Remeta, D.P. and Minetti, C.A. (2007) What drives proteins into the major and minor grooves of DNA. Biopysical Journal, Suppl. S, 231A-231A.

 
  1. Privalov, P.L. & Dragan, A.I. (2007) Microcalorimetry of biological macromolecules. Biophys Chem. 126(1-3), 16-24.

 
  1. Serebruany, V., Malinin, A., Dragan, V.A., Atar, D., van Zyl, L., Dragan, A. I. (2007) Fluorimetric quantitation of citalopram and escitalopram in plasma: developing an express method to monitor compliance in clinical trials.
    Clin Chem Lab Med. 45(4), 513-20.

 
  1. McDonald, R.J., Dragan, A.I., Kirk, W.R., Neff, K.L., Privalov, P.L. & Maher, L.J. (2007) DNA bending by charged peptides: electrophoretic and spectroscopic analyses. Biochemistry 46(9), 2306-16.

 
  1. Dragan, A.I., Makeeva, E.N. and Privalov, P.L. (2007) Mechanisms of activation of the IRF transcription factor: The role of the C-terminal domain phosphorylation in the IRF-3 dimerization and DNA binding. Biophysical Journal, Suppl. S, 55A-55A.

 
  1. Privalov, P.L., Dragan, A.I., Crane-Robinson, C., Breslauer, K.J., Remeta, D.P. & Minetti, C.A. What drives proteins into the major or minor grooves of DNA?
    J Mol Biol. 2007 Jan 5;365(1), 1-9.

 
  1. Crane-Robinson, C., Dragan, A.I. & Privalov, P.L. (2006) The extended arms of DNA-binding domains: a tale of tails. Trends Biochem Sci. 31(10), 547-52.

 
  1. Dragan, A.I., Li, Z., Makeyeva, E., Milgotina, E., Liu, Y., Crane-Robinson, C. & Privalov, P.L (2006) Forces driving the binding of homeodomains to DNA. Biochemistry, 45, 141-151.

 
  1. Hargreaves, V.V, Makeyeva, E.N., Dragan. A.I. & Privalov, P.L  (2005) Stability and DNA binding ability of the DNA binding domains of interferon regulatory factors 1 and 3. Biochemistry, 44, 14202-14209.

 
  1. Oshrine B, Malinin A, Pokov A, Dragan A, Hanley D, Serebruany V. (2005) Criticality of pH for accurate fluorometric measurements of dipyridamole levels in biological fluids. Methods Find Exp Clin Pharmacol., 27(2), 95-100.

 
  1. Dragan A.I., Frank L., Liu Y., Makeyeva E. N.,  Crane-Robinson C. & Privalov P.L. (2004) Thermodynamic signature of GCN4-bZIP binding to DNA indicates the role of water in discriminating between the AP-1 and ATF/CREB sites. J.Mol. Biol. 343, 865-878.

 
  1. Dragan, A.I., Read, C.M., Makeyeva, E.N., Crane-Robinson, C. and Privalov, P.L. (2004) DNA binding and bending by sequence specific HMG boxes: Energetic eeterminants of specificity. Biophysical Journal, 86 (1), 623A-623A.

 
  1. Dragan, A.I., Potekhin, S.A., Sivolob, A.V., Lu M. and Privalov, P.L. (2004) Kinetics and Thermodynamics of the Unfolding and Refolding of the Three-Stranded alpha-Helical Coiled Coil, Lpp-56. Biochemistry 43(47), 14891-14900.

 
  1. Dragan A.I., Read C. M., Makeyeva E. N., Milgotina E. I., Churchill M.E.A.,Crane-Robinson C. & Privalov P.L. (2004) DNA binding and bending by sequence specific HMG boxes: energetic determinants of specificity. J. Mol. Biol. 343, 371-393.

 
  1. Dragan A.I., Liu Y., Makeyeva E. N. & Privalov P.L. (2004) GCN4-bZIP induces bending of both the ATF/CREB and AP-1 binding sites of DNA. Nucleic Acids Res. 32, 5192-5197.

 
  1. Dragan A.I., Klass J., Read C., Churchill M.E.A., Crane-Robinson C. & Privalov P.L.    (2003) DNA binding of a non-sequence-specific HMG-D protein is entropy driven with a substantial non-electrostatic contribution. J. Mol. Biol. 331, 795-813.

 
  1. Dragan, A.I., Liggins J.R., Crane-Robinson C. & Privalov P.L. (2003) The energetics of specific binding of AT-hooks from HMGA1 to target DNA. J.Mol.Biol. 327, 393-411.

 
  1. Dragan, A.I. & Privalov P.L. (2002) Unfolding of a leucine zipper is not a simple two-state transition. J.Mol.Biol. 321, 891-908.

 
  1. Privalov, P.L., Jelesarov, I., Read, C.M., Dragan, A.I.& Crane-Robinson, C. (1999)  The energetics of HMG box interactions with DNA: thermodynamics of the DNA binding of the HMG box from mouse Sox-5. J. Mol. Biol. 294, 997-1013.

 
  1. Crane-Robinson, C., Read, C.M., Cary, P.D., Driscoll, P.C., Dragan, A.I. & Privalov, P.L. (1998) The energetics of HMG box interactions with DNA. Thermodynamic description of the box from mouse Sox-5. J. Mol. Biol. 281, 705-717.

 
  1. Khrapunov, S.N., Dragan, A.I., Sivolob, A.V. & Zagaria, A.M.(1997) Mechanisms of stabilizing nucleosome structure. Study of dissociation of the histone octamer from DNA. Biochim. Biophys. Acta. 1351, 213-222.

 
  1. Khrapunov,  S.N. & Dragan, A.I. (1996)  To the question of mutagenic action of laser radiation. Cytology and genetics. 30, 58 - 65.

 
  1. Dragan, A.I.&  Khrapunov, S.N. (1994) Reaction of fluorescamine  with nitrogenous bases   as   part  of  the  nucleotides  and  single-strand synthetic and                   natural oligonucleotides. Biophysics. 39, 267-271.

 
  1. Khrapunov, S.N. &  Dragan,.A.I. (1994) Chromosome aberrations and DNA photocleavages induced  by  laser.  Mechanisms and specifity. In: Plant biotechnology and genetics  engineering.  Kiev, p19-20.

 
  1. Khrapunov, S.N.,  Dragan, A.I.,  Pavlic, B.D. &  Zubrilin, N.G. (1994) Molecular mechanisms  of  DNA  photocleavaging  by laser. In: Laser Applications in life sciences, Minsk, p.108-109.

 
  1. Dragan, A.I. & Khrapunov,  S.N. (1994)  Molecular  mechanisms  of  DNA damage by  laser  radiation.  Mol.  Biol. 28, 239-243.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1993)  About the mechanisms of cytogenetic action of laser radiation. Cytology and genetics, 27, 20 - 24.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1993)  Determination of quantum yields of photocleavage of DNA by laser in the presence of intercalated and nonintercalated dyes. Biopolymers and Cell. 9(4), 22-25.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1992)  Non-linear effects of laser radiation action on DNA-Dye complexes. Proc. Acad. Sci. Ukraine. 12,115-118.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1992) Study of mutagenic action of laser radiation on A. fistulosum.   Cytology and Genetics. 26, 32-36.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1989)  Absorbtion and luminescence studies of intermolecular interactions of tyrosine chromophore. I. Analysis of absorbtion and fluorescence spectra.  Biophysics. 34 (1), 7-13.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1989)  Absorbtion and luminescence studies of intermolecular interactions of tyrosine chromophore. II. Influence of solvent polarity on the fluorescence spectra of chromophore. Biophysics. 34, 187-191.

 
  1. Dragan, A.I. &  Khrapunov,  S.N. (1989)  Spectroscopy of intermolecular interactions of a tyrosine chromophore. III. Classification of the state of tyrosine residues in proteins based on their electron spectra. Biophysics. 34, 357-363.

 
  1. Khrapunov, S.N., Kadura, S.N.& Dragan, A.I. (1988) Rearrangements of   chromatin   structure   during  spermatogenesis  of  squid. Eur.J.Biochem. 175, 609-613.

 
  1. Dragan, A.I. & Khrapunov, S.N. (1987) New methodical approaches to the study of the basic and excited states of the tyrosine chromophores. Mol. Gen. and Biophys., 12, 103-108.

 
  1. Dragan, A.I., Sivolob, A.V. & Khrapunov, S.N. (1987) The nature of forces stabilizing the structure of the nucleosome. Investigation of the process of the histone octamer dissociation from DNA. Mol. Biol., 21,724-736.

 
  1. Khrapunov, S.N., Kadura, S.N.& Dragan, A.I. (1987) Structural rearrangements of   chromatin during  spermatogenesis  of  the squid Illex-Argentinus. Biochemistry. 52, 1232-1239.

 
  1. Sivolob, A.V., Dragan, A.I. & Khrapunov, S.N. (1987) Theoretical study of structural transition of the nucleosome at low ionic strength. Mol. Biol., 21, 714-723.

 
  1. Khrapunov, S.N., Sivolob, A.V., Dragan, A.I. & Berdyshev, G.D. (1985) Structure of histone octamer within reconstructed polynucleosomes. Mol. Biol., 19, 1262-1269.

 
  1. Dragan, A.I. Khrapunov, S.N., & Berdyshev, G.D. (1985) Analysis of the equilibrium of histone oligomers in solution. The nature of forces stabilizing the (H2A-H2B-H3-H4)2 octamer structure. Mol. Biol., 19, 1031-1039.

 
  1. Khrapunov, S.N., Protas, A.F., Dragan, A.I., & Berdyshev, G.D. (1985) Spatial organization of the (H2A-H2B-H3-H4)2 histone octamer. Mol. Biol., 19, 833-841.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F. & Berdyshev, G.D. (1985) Two structural forms of histone octamer. Biophysics, 30(5), 791-796.

 
  1. Khrapunov, S.N., Sivolob, A.V., Protas, A.F., Dragan, A.I. & Berdyshev, G.D. (1985) Intrinsic fluorescence, difference spectrophotometry and theoretical studies on tertiary structure of calf thymus histone H1. Int. J. Biochem., 17, 217-222.

 
  1. Savluk, O.S., Deinega, E.Y., Dragan, A.I. & Kulsky, L.A. (1985) Mechanizm of the static electric field action on the water desinfection processes. Proc. Ac. Sci. Ukraine. 4, 70-73.

 
  1. Dragan, A.I. (1985) The structure of the isolated histone proteins and their aggregates in model experiments. In: Structure and genetic meaning of the eucaryotic chromatin proteins. Publisher: Science Express, Inc., Kiev.

 
  1. Khrapunov, S.N., Protas, A.F., Sivolob, A.V., Dragan, A.I. & Berdyshev, G.D. (1984) Characteristics of tertiary structure of histone H1 molecule from calf thymus. Mol. Biol. 18, 794-801.

 
  1. Protas, A.F., Khrapunov, S.N., Dragan, A.I. & Berdyshev, G.D. (1984) Investigation of native and trypsin treated oligomers of histones: the tetramer (H3-H4)2 and dimer (H2A-H2B). Biochemistry. 49, 642 – 646.

 
  1. Khrapunov, S.N., Protas, A.F., Dragan, A.I. & Berdyshev, G.D. (1984) Spatial organization of the histone dimer H2A-H2B in solutions with different ionic strengths. Mol. Biol. 17, 807-814.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F., & Berdyshev, G.D. (1984) Structure of the histone tetramer (H3-H4)2: 1. Peculiarities of optical absorption, fluorescence and light scattering in response to changes in ionic strength and pH of the medium. Int. J. Biol. Macromol. 6, 26-30.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F., & Berdyshev, G.D. (1984) The structure of the histone dimmer H2A-H2B studied by spectroscopy. Biochimica et Biophysica Acta. 787, 97-104.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F., & Berdyshev, G.D. (1984) Structure of the histone tetramer (H3-H4)2: 2. Position of lambda max in the tyrosyl fluorescence spectra and tyrosyl accessibility to quenchers. Int. J. Biol. Macromol. 6, 31-34.

 
  1. Khrapunov, S.N., Dragan, A.I., Sivolob, A.V., Kadura, S.N. & Berdyshev, G.D. (1983) Peculiarities of the amino acid composition, spacial organization and interaction with DNA of histones H1 from calf thymus and carp spermatozoa. Biochemistry. 48, 926-934.

 
  1. Dragan, A.I., Khrapunov, S.N., Protas, A.F., & Berdyshev, G.D. (1983) The change in maximum position of tyrosyl fluorescence spectra of RNase A and histone dimer (H2A-H2B) under denaturation. Studia Biophysica. 96, 187-193.

 
  1. Dragan, A.I., & Khrapunov, S.N. (1983) The red shift of tyrosine fluorescence spectrum in polyethylenglycol and urea solutions. Studia Biophysica. 96, 127-132.

 
  1. Protas, A.F., Khrapunov, S.N., Dragan, A.I., & Berdyshev, G.D. (1983)  Isolation and physicochemical characteristics of native histone complexes – dimmer (H2A-H2B), tetramer (H3-H4)2 and octamer (H3-H4-H2A-H2B)2. Ukr. Biochem. J. 55, 136-140.

 
  1. Dragan, A.I., Khrapunov, S.N., Protas, A.F., & Berdyshev, G.D. (1983) Registration of the stuctural transitions in tyrosine-containing proteins by fluorescent spectroscopy methods. Review. In: “Luminescence analysis in medico-biological research”. Publisher: RMI, p.204-211.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F. and Berdyshev, G.D. (1983) The spatial organization of the (H2A-H2B) histone dimer in solution with high ionic strength. Mol. Biol. 17, 992-999.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F. and Berdyshev, G.D. (1983) Structure of the histone tetramer (H3-H4)2: I. Peculiarities of optical absorption, fluorescence and light scattering in response to changes of ionic strength and pH of the medium. Int. J. Biol. Macromol., 6, 26-30.

 
  1. Khrapunov, S.N., Dragan, A.I., Protas, A.F. and Berdyshev, G.D. (1983) Structure of the histone tetramer (H3-H4)2: II. Position of max in the tyrosyl fluorescence spectra and tyrosyl accessibility to quenchers. Int. J. Macromol., 6, 31-34.

 
  1. Khrapunov, S.N., Berdyshev, G.D., Zyma, V.L. and Dragan, A.I. (1979)Study of the structure and aggregation of the histones. IV. Change in tertiary structure of histone H2A in solutions with high ionic strength. Biophysics. 24, 565-566.

 
  1. Khrapunov, S.N., Berdyshev, G.D., & Dragan, A.I. (1978) Investigation of histones structure and aggregation. 2. Structure of oligomers forming in mixed solutions of histones H2A and H4. Biophysics. 23, 995-999.

 
  1. Zyma, V.L., Dragan, A.I. & Bogach, P.G. (1978) Fluorescence and thermal perturbation difference spectra of tryptophan in hydrophobic environment. Proc.Acad.Sci.Ukraine. 11, 1016-1019.

 

Presentations, Conferences, Scientific Meetings, Symposia:

 

  1. Pavlovic, R., Barannikova, E., Mali, B., Dragan, A.I. and Geddes, C.D. Advanced Plasmonic Surfaces for Metal-Enhanced Fluorescence (MEF). MARM 2012, ACS Middle Atlantic Regional Meeting, Baltimore, USA, May 31 – June 2, 2012.

 
  1. Karolin, J., Dragan, A.I. and Geddes, C.D. Metal-Enhanced Fluorescence (MEF): Developing a Unified Fluorophore Plasmon Model. EMRS 2012, European Materials Research Society, Srtasbourg, France, May 14 – 18, 2012.

 
  1. Mali, B., Dragan, A.I., Barannikova, E. and Geddes, C.D. Au-fluorescent labeling of proteins and their photophysical characterization. A Look Ahead XV (UMBC): Futures in Biomedical Research, April 18, 2012.

 
  1. Dragan, A.I., Barannikova, E. and Geddes, C.D. Metal-enhanced chemiluminescence (MEC) from advanced plasmonic surfaces. A Look Ahead XV (UMBC): Futures in Biomedical Research, April 18, 2012.

 
  1. Pavlovic, R., Dragan, A.I., and Geddes, C.D. Multi-color microwave accelerated metal-enhanced fluorescence DNA technologies. A Look Ahead XV (UMBC): Futures in Biomedical Research, April 18, 2012.

 
  1. Dragan, A.I., Pavlovic, R., Melendez, J. and Geddes, C.D. Ultra-fast and ultra sensitive multiplexed pathogen detection. MARCE 2012 RCE National Meeting, Amelia Island, Florida, USA, March 26 – 28, 2012.

 
  1. Dragan, A.I., Mali, B. and Geddes, C.D. Luminescence of Au-protein complexes: metal-enhanced fluorescence, delayed emission and microwave-based preparation. 56th Annual Meeting of Biophysical Society, San-Diego, California, USA, February 25 – 29, 2012.

 
  1. Dragan, A.I., Barannikova, E., Mali, B. and Geddes, C.D. Metal-Enhanced Fluorescence Excitation Volumetric Effect (MEF EVE). 56th Annual Meeting of Biophysical Society, San-Diego, California, USA, February 25 – 29, 2012.

 
  1. Feng, C., Whitford, M., Stamatos, N., Cole, L., Medvedev, A., Dragan, A.I., Geddes, C. and Cross, A. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. Gordon conference on Glycobiology, Barga, Italy, May 11, 2011.

 
  1. Dragan, A.I., Pavlovic, R. and Geddes, C.D. Rapid Catch and Signal (RCS) platform technology: Multiplexed three-color, microwave-accelerated metal-enhanced fluorescence 20 second DNA assays. Biophysical Society Meeting, Baltimore, March 2011.

 
  1. Dragan, A.I., Pavlovic, R. and Geddes, C.D. Anthrax PA exotoxin detection assay based on Micriwave Accelerated Metal-Enhanced Fluorescence: ultra-fast and sensitive. Symposium UMBC: A Look Ahead, Baltimore, April 2011.

 
  1. Zhang, Y., Dragan, A.I. and Gededs, C.D. Metal-Enhanced Fluorescence: theory and applications. Symposium UMBC, Baltimore, March 2010.

 
  1. Dragan, A.I., Bishop, E.S., Casas-Finet, J.R., Strouse, R.J., Schenerman, M.A. and Geddes, C.D. Metal_Enhanced PicoGreen Fluorescence for sensitive DNA quantitation. 14th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products. WCBP 2010, Washington, January 2010.

 
  1. Dragan, A.I., Geddes, C.D. Plasmonic Electricity: A Digital form of Metal‐Enhanced Fluorescence. Abstract. Biophysical Society 53-rd Annual Meeting, Boston, March 2009.

 
  1. Dragan, A.I., Geddes, C.D. Characterization of PicoGreen binding to dsDNA and the origin of its fluorescence enhancement upon intercalation between DNA base pairs. Presentation. MedImmune LLC, March 2009.

 
  1. Dragan, A.I., Geddes, C.D. Metal-Enhanced Fluorescence (MEF) of PicoGreen in Complex with DNA. Presentation. MedImmune LLC, October 2008.

 
  1. Dragan, A.I. Calorimetry of Biopolymers. Thermodynamic Insight on Protein-DNA Interactions. Presentation. The Dow Chemical Company, April 2008.

 
  1. Privalov, P.L., Dragan, A.I., Crane-Robinson, C., Breslauer, K.J., Remeta, D.P. & Minetti, C.A. What drives proteins into the major or minor grooves of DNA? Presentation. Biophysical Society 51-rd Annual Meeting, Baltimore, March 2007.

 
  1. Dragan, A.I., Privalov, P.L. Assembling the human IFN-beta enhanceosome in solution. Presentation. Biophysical Society 51-rd Annual Meeting, Baltimore, March 2007.

 
  1. Dragan A.I., Read C. M., Makeyeva E. N., Milgotina E. I., Churchill M.E.A.,Crane-Robinson C. & Privalov P.L. DNA binding and bending by sequence specific HMG boxes: energetic determinants of specificity. Presentation. Biophysical Society 48-rd Annual Meeting, Baltimore, February 2004.

 
  1. Dragan, A.I., Lu, M., Potekhin, S.A., Privalov, P.L. Kinetics and thermodynamics of formation of the tri-meric alpha-helical coiled-coil, Lpp-56. The Six Johns Hopkins Folding Meeting, Coolfont, Virginia, March 2001, Abstract, p.17.

 
  1. Dragan, A.I., Lu, M., Privalov, P.L. Folding and Refolding of the three-stranded coiled-coil. Thermodynamic and kinetic study. Inth Annual Retreat, Institute for Biophysical Research, USA, 27-28 October 2000.

 
  1. Khrapunov, S.N., Dragan, A.I., Sivolob, A.V. The topological complementarity of the charge groups controlling the interaction of the DNA with core histones in the nucleosome. XII-th International Biophysics Cngress. Amsterdam, The Netherlands, 11-16 August 1996. Progress in Biopysics and Molecular Biology, 65, 82-97.

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Дизайн: Інститут високих технологій
Ivan Ivanov