Development and optimization of bi-enzyme conductometric biosensor for L-arginine determination

Назва конференції: 
Journee Rhone-Alpes des Biomolecules
Назва конференції (тільки ім'я): 
Journee Rhone-Alpes des Biomolecules
Номер конференції: 
-
Країна: 
France
Місто: 
Lyon
Сторінки: 
1
Кількість сторінок: 
1
Вихідні данні тез: 
Talanta 92.- P.58-64 2012
Рік: 
2011
Дата проведення: 
27.05.2011
Автор(и): 
O.Y.Saiapina, N.Jaffrezic-Renault, S.V.Dzyadevych
Мова: 
Англійська
Назва підрозділу: 
Кафедра молекулярної біології, біотехнології та біофізики
A highly sensitive conductometric biosensor for l-arginine determination was developed by exploiting the unique biorecognition capacities of two enzymes of urea cycle - arginase (E.C. 3.5.3.1) and urease (E.C. 3.5.1.5). The enzymes were co-immobilized in a single bioselective membrane on the working sensor, while a lysine rich bovine serum albumin (BSA) membrane was immobilized on the reference sensor, allowing differential measurements. The optimum percentage ratio of arginase and urease within the bioselective membrane was determined when the biosensor sensitivity to l-arginine and urea was optimum. Analytical characteristics of the conductometric biosensor for l-arginine determination were compared for two types of enzyme immobilization (cross-linking with glutaraldehyde (GA) and entrapment in the polymeric membrane). The optimum features in terms of the sensitivity, the linear range, and the detection limit (4.2 μS/mM, 0.01-4mM, and 5.0 × 10(-7)M, respectively) were found for l-arginine biosensor based on enzyme cross-linking with GA. A quantitative determination of l-arginine in the real sample (a drinkable solution "Arginine Veyron") gave a satisfactory result compared to the data provided by the producer (a relative error was 4.6%). The developed biosensor showed high operational and storage stability.
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Дизайн: Інститут високих технологій
Ivan Ivanov